Garry Nolan, PhD, Rachford and Carlota A. Harris Professor, Department of Pathology, School of Medicine, Stanford University

Research Description: Dr. Nolan’s current research is focused on the development of multiparameter, singlecell tissue imaging technologies, and computational approaches for systems immuno-oncology. Two recent innovations from his lab include multiplexed ion beam imaging (MIBI) and CO-Detection by indEXing (CODEX), which allow for simultaneous spatial quantification of 50+ parameters in a single tissue section. Another focus of his lab is the development and utilization of machine learning algorithms to interpret the large, high-dimensional datasets produced by CyTOF, multiplexed ion beam imaging (MIBI), and CODEX. Collectively, the efforts are to provide a deeper understanding of normal and impaired immune function - including detailed substructures of the immune system as it relates to various cancerous states - to enable wholly new understandings that lead to improved clinical outcomes. CyTOF and CODEX reagents have both been adapted towards Obesity research with antibodies aimed at metabolism and fat cell precursors in recent efforts in his lab and those of former mentees such as Sean Bendall at Stanford University. Current interest is to extend the CODEX technologies to metabolic tissue and focus on how adipose tissue and gut show activation of inflammation as a response to obesity. His work is in conjunction with the Jackson lab. Using secretomics, they are working to further identify the key factors that drive inflammation in fat and gut and use CODEX technology to correlate locally secreted factors with the recruitment of inflammatory and immune cells. Dr. Nolan collaborates extensively with many members of the NORC.